Organ development in multicellular systems is governed by the activation of a new genetic program and the repression of a previously active program, which is largely governed by epigenetic systems.
National Key Research and Development Program (2018YFD1000500) Funding had role in study design and material collection the Natural Science Foundation of Hainan Province (320QN193) had role in the research of qPCR data collection and analysis China Agriculture Research System (CARS-11-hncyh) founders had role in RNA-Seq data collection and analysis, manuscript preparation.Ĭompeting interests: The authors have declared that no competing interests exist. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: All relevant data are within the manuscript and its Supporting Information files.įunding: This study was supported by National Key Research and Development Program (2018YFD1000500), the Natural Science Foundation of Hainan Province (320QN193) and China Agriculture Research System (CARS-11-hncyh). Received: FebruAccepted: FebruPublished: April 20, 2023Ĭopyright: © 2023 Mao et al. PLoS ONE 18(4):Įditor: Vibhav Gautam, Banaras Hindu University, INDIA (2023) Genome-wide analyses of LATERAL ORGAN BOUNDARIES in cassava reveal the role of LBD47 in defence against bacterial blight. These findings provided a comprehensive analysis of ASL/LBD genes and laid the groundwork for future research to understand ASL/LBD genes.Ĭitation: Mao Y, Abdoulaye AH, Song J, Yao X, Zhang Y, Gao Y, et al. The result indicated that MeASLBD47 significantly mitigated the virulence of cassava bacterial blight ( XamCHN11) through Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) and Virus-induced gene silencing (VIGS). The MeASLBD47 gene was selected for functional analysis. The transcriptome data of cassava under biotic and abiotic stresses revealed that MeASLBD46 and MeASLBD47 greatly respond to disease and drought.
Furthermore, the analysis of cis-regulatory elements in promoter regions suggested that MeASLBDs may be involved in the plant phytohormone signal response. Weighted gene co-expression network analysis (WGCNA) of target genes and promoter analysis suggest that these MeASLBDs may be involved in hormone and stress responses.
The gene structure and motif composition were conserved in MeASLBDs, while the expression profiles of these genes were highly diverse, implying that they are associated with diverse functions. Characterisation and identification of cassava ASL/LBD genes using the computational algorithms, hidden Markov model profiles (PF03195), determined 55 ASL/LBD genes ( MeASLBD1 to MeASLBD55). Nevertheless, it remains uncharacterised in cassava ( Manihot esculenta). AS2/LOB domain has been characterised in plants such as A. AS2/LOB domain consists of an amino-terminal (N-terminal) that contains a cysteine repeat (the C-motif), a conserved glycine residue, and a leucine-zipper-like. The AS2 gene belongs to the plant-specific AS2-LIKE/LATERAL ORGAN BOUNDARIES ( LOB)-domain ( ASL/LBD), which consists of 42 proteins in Arabidopsis with a conserved amino-terminal domain known as the AS2/LOB domain, and a variable carboxyl-terminal region. The Arabidopsis thaliana ASYMMETRIC LEAVES2 ( AS2) gene is responsible for the development of flat, symmetric, and extended leaf laminae and their veins.
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